研究目的
The study aims to demonstrate the efficacy of green nanomedicine, specifically gold nanoparticles obtained through green chemistry, in the plasmonic thermal destruction of surface cancers. It focuses on optimizing tumor microenvironment targeting and determining the specific irradiation dose required for effective treatment.
研究成果
The study successfully demonstrated the proof of concept for the plasmonic photothermal treatment of surface and surgical access cancers using green nanogold theranostic. It emphasized the importance of noninvasive destruction of cancer cells with a single nontoxic dose activated after intratumoral injection by non-ionizing irradiation.
研究不足
The study highlights the need for personalized physical parameters of plasmonic photothermal therapy (PPTT) to induce irreversible damages without causing necrosis of healthy tissue. It also points out the challenge of generating optimal hyperthermia in vitro compared to in vivo conditions due to the absence of the microenvironment.
1：Experimental Design and Method Selection:
The study utilized a greener approach for designing surface plasmon resonant gold nanoparticles using a hydrosoluble fraction of an endemic asteraceae medicinal plant. The nanoparticles were characterized and their efficacy was assessed in vitro and in vivo on murine models.
2：Sample Selection and Data Sources:
The study used tumor cell lines derived from surgical explants of pancreas carcinoma and malignant melanoma. Biodistribution studies were conducted on BALB/c nude male mice.
3：List of Experimental Equipment and Materials:
Equipment included a Perkin Elmer Lambda UV/Vis 950 spectrophotometer, TEM/STEM Technai Osiris microscope, Zetasizer NanoZSP, and ThermoFisher Scientific K-ALPHA spectrometer. Materials included tetrachloroauric acid, EDC, NHS, carbohydrate, and peptide.
4：Experimental Procedures and Operational Workflow:
Gold nanoparticles were synthesized, characterized, and functionalized. In vitro and in vivo assessments of their biological activity and photothermal therapy efficacy were conducted.
5：Data Analysis Methods:
Data was analyzed by One-Way Analysis of Variance (ANOVA) followed by post-hoc comparisons by Tukey’s HSD test.
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