摘要： Purpose: To determine the effect of aging on the corneal subbasal nerve plexus (SNP) by employing a wide-field mapping technique of composite images, scanned at the location of a distinctive spiraled subbasal nerve pattern located 1 to 2 mm inferior to the corneal apex (the inferior whorl) for SNP structural quantification. Material and methods: The central corneal tactile sensitivity (CCTS) and inferior whorl length (IWL) were compared among individuals in three age groups (20–39 years, 40–59 years, and 60–79 years). Statistical analyses constituted the Kruskal-Wallis test, one-way analysis of variance (with post hoc least significant difference test), Spearman correlation coefficient, and linear regression. Results: CCTS remained stable until the age of 50 years, when it began to decrease; the mean CCTS was 58.15±2.46 mm in the group aged 20–39, 55.74±3.85 mm in the group aged 40–59, and 50.23±3.27 mm in the group aged 60–79. IWL decreased with increasing age, with a corresponding linear decline of 0.2088 mm/mm2 per year, and the mean IWL was 25.43±4.50 mm/mm2 in the group aged 20–39, 22.71±6.19 mm/mm2 in the group aged 40–59, and 18.60±4.21 mm/mm2 in the group aged 60–79. Conclusion: Our work provided a more accurate and repeatable method for corneal nerve analysis using Laser-Scanning Confocal Microscopy (LSCM). By using this technique, we confirmed that aging is associated with progressive reduction in subbasal nerve length.