研究目的
To study the proliferation of cartilaginous tissue cells (chondroblasts) and mesenchymal stem cells (MSCs) on the surface of synthesized MWCNT arrays and compare the processes of chondroblast growth on pure silicon and MWCNT arrays.
研究成果
MWCNT arrays synthesized on a silicon substrate support higher cell proliferation density compared to pure silicon. Laser structuring makes the arrays resistant to bending under the influence of nutrient medium with stem cells, without toxic or pathological effects on cell viability and morphology. These findings suggest potential applications in biomedical devices.
研究不足
The study focuses on the proliferation of chondroblasts and MSCs on MWCNT arrays and does not explore the long-term effects or potential applications in vivo. The scalability of the laser structuring method for larger substrates may require further optimization.
1：Experimental Design and Method Selection:
MWCNT arrays were synthesized by catalytic plasma-enhanced chemical vapor deposition. A method for structuring the MWCNT arrays by 100-ns laser pulse scanning and abrasive water processing was developed.
2：Sample Selection and Data Sources:
Chondroblasts isolated from human articular hyaline cartilage and horse mesenchymal stem cells were used.
3：List of Experimental Equipment and Materials:
Oxford PlasmaLab System 100 for MWCNT synthesis, SANYO incubator for cell incubation, and a 1064-nm nanosecond ytterbium fiber laser for structuring.
4：Experimental Procedures and Operational Workflow:
Cells were cultured on MWCNT arrays and pure silicon substrates, incubated, and then prepared for SEM.
5：Data Analysis Methods:
SEM images were analyzed to compare cell proliferation and morphology on different substrates.
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