研究目的
To describe a simple method using a laser pointer to detect dirt and damage in light microscopy objective lenses that may not be visible during routine maintenance.
研究成果
A laser pointer can be used to detect dirt and damage in light microscopy objective lenses, revealing damage unnoticed by other means when cleaning and visual inspection fail to show any irregularity in a poor performing objective.
研究不足
The exact nature of the effect observed with the laser pointer is not completely clear, and the method may require further validation to distinguish between different types of lens damage or dirt.
1：Experimental Design and Method Selection:
The study employs a laser pointer to inspect objective lenses by projecting the light exiting the lens onto a flat surface to identify damage or dirt.
2：Sample Selection and Data Sources:
The method was tested on a 100×, achromat, oil-immersion objective from a Leica light microscope used in teaching clinical microbiology.
3：List of Experimental Equipment and Materials:
A common laser pointer (JD-303, 532 nm, 20 mW), a Leica light microscope model CME, and a white wall for projection.
4：Experimental Procedures and Operational Workflow:
The laser pointer was aligned with the front lens of the objective, and the light exiting the reverse aperture was projected onto a white wall to observe the image produced.
5：Data Analysis Methods:
Visual inspection of the projected images to identify patterns indicative of lens damage or dirt.
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