研究目的
Developing a rapid method to quantify and characterize the oligomeric fraction of bio-oils in terms of molar mass using SEC-MALS-DRI.
研究成果
The SEC-MALS-DRI method allows rapid PL quantification and characterization in terms of molar mass, providing a simpler alternative to the dropwise water precipitation method. The condensation system and the water precipitation method were found to affect the average molar mass of isolated PL oligomers.
研究不足
The study highlighted the need for careful selection of the integration interval and the precise value of the dn/dc parameter for accurate PL quantification. The SEC separation parameters could be optimized to minimize peak overlapping.
1：Experimental Design and Method Selection:
The study used size exclusion chromatography (SEC) coupled with differential refractive index (DRI) and multi-angle laser light scattering (MALS) detectors to characterize the Pyrolytic Lignin (PL) fraction of bio-oils.
2：Sample Selection and Data Sources:
Two beech wood bio-oils with different PL content were produced using two condensation systems: a standard and a fractional condensation chain.
3：List of Experimental Equipment and Materials:
A Shimadzu HPLC system equipped with a pump, a solvent degasser, an auto sampler, and a SEC-column placed in an oven, kept at 45 °C. The flow-rate of the mobile phase was fixed at
4：65 mL/min. Experimental Procedures and Operational Workflow:
PL was isolated from bio-oils by the water precipitation method. The PL content was estimated from the SEC-MALS-DRI method and compared to the PL content determined by the precipitation method.
5：Data Analysis Methods:
Data acquisition and calculations were performed with ASTRA software version 6.1.7.16 (Wyatt technology).
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