研究目的
Rapid onsite screening for Rhodamine B (RhB) in food without sample pretreatment using solid-phase extraction (SPE) coupled with direct fluorescence detection.
研究成果
The proposed method is a valid rapid screening method for RhB, combining SPE with direct on-substrate fluorescence sensing in food safety screening. It offers simplicity, short analysis time, low detection limit, and the use of inexpensive equipment.
研究不足
The method is qualitative or semi-quantitative and suitable for onsite analysis but not for accurate quantification. The sensitivity and reliability are limited by the quantity of analytes recovered.
1:Experimental Design and Method Selection:
The study proposed a method combining SPE with direct fluorescence detection for RhB screening in food.
2:Sample Selection and Data Sources:
RhB-spiked chili powder, preserved plums, and sausages were used as test samples.
3:List of Experimental Equipment and Materials:
PVA hydrogel was prepared using PVA, sodium tetraborate, and RhB. Fluorescence spectra were collected using a spectrofluorimeter, and HPLC was used for validation.
4:Experimental Procedures and Operational Workflow:
The PVA hydrogel was used to extract RhB from food samples, followed by fluorescence detection.
5:Data Analysis Methods:
The fluorescence intensity was measured to determine RhB concentration, with HPLC used for verification.
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HPLC
1260 LC-FLD
Agilent
Used for comparison and validation of RhB in spiked food samples
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PVA
MW = 89,000–98,000, 99+% hydrolyzed
Sigma-Aldrich
Used to prepare PVA hydrogel for solid-phase extraction of RhB
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sodium tetraborate
99%
Sigma-Aldrich
Used in the preparation of PVA hydrogel
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spectrofluorimeter
F-7000
Hitachi
Used to collect fluorescence spectra of RhB
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rhodamine B
Sigma-Aldrich
Analyte for detection in food samples
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universal grinder
FW100
Kewei
Used for grinding the samples
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vortex
VORTEX 3
IKA
Used to improve the extraction efficiency for HPLC analysis
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ultrasound cleaner
D9
GT-SONIC
Used to improve the extraction efficiency for HPLC analysis
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