研究目的
Investigating the development of novel fluorescent sensors based on the combination of an environment─ sensitive fluorophore with polymeric and self─ assembled architectures for sensitive temperature and pH measurements in biological and medical studies.
研究成果
The study successfully developed highly sensitive fluorescent polymeric thermometers and digital fluorescent pH sensors based on a novel functional design. These sensors have been applied in intracellular temperature measurements and nanoscale proton mapping, demonstrating the potential for advanced biological and medical applications.
研究不足
The study focuses on synthetic systems and their application in controlled environments. The practical application in complex biological systems may require further optimization for stability and specificity.
1：Experimental Design and Method Selection:
The study involves the design and synthesis of fluorescent sensors with environment─ sensitive fluorophores and stimulus─ responsive polymers. The fluorescence properties are tuned by controlling the ICT character and PET efficiency.
2：Sample Selection and Data Sources:
The study uses synthetic fluorescent probes for ions and molecules, and thermoresponsive polymers like PNIPAM.
3：List of Experimental Equipment and Materials:
Includes fluorophores (e.g., benzofurazan), polymers (e.g., PNIPAM), and radical initiators (e.g., AIBN).
4：Experimental Procedures and Operational Workflow:
Synthesis involves mixing monomers and a radical initiator, removing dissolved oxygen, and purifying the product. Fluorescence properties are measured with increasing temperature or pH.
5：Data Analysis Methods:
Fluorescence intensity, wavelength, and lifetime are analyzed to determine temperature or pH sensitivity.
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