研究目的
Investigating the repeatability and reproducibility of bovine methane emissions measured using a laser methane detector to assess its potential as a tool for large-scale studies.
研究成果
The laser methane detector shows promise as a tool for measuring bovine CH4 emissions in field conditions, with log-transformed data providing better repeatability and reproducibility. The number of CH4 peaks could serve as a useful phenotype to differentiate between high and low emitting animals.
研究不足
The study was conducted on a small sample size of three heifers, which may not represent the variability in larger populations. Environmental factors and animal behavior during measurements could affect the accuracy of CH4 emission records.
1:Experimental Design and Method Selection:
The study used a laser methane detector to measure CH4 emissions from Simmental heifers in a commercial farm setting. Measurements were taken five times a day for three consecutive days.
2:Sample Selection and Data Sources:
Three pregnant Simmental heifers of varying weights and ages were selected.
3:List of Experimental Equipment and Materials:
Laser Methane Mini (Crowcon, Abingdon, UK) was used for measurements. Data was forwarded via Bluetooth to a Lenovo Tab E7 tablet equipped with Gas Viewer application.
4:Experimental Procedures and Operational Workflow:
Each measurement involved pointing the laser at the nostril of a heifer for 5 minutes at a distance of 3 meters. Data was collected every
5:5 seconds. Data Analysis Methods:
Data was log-transformed to achieve normality. Repeatability and reproducibility were calculated as the relative standard deviation of measurements within the same day and across three days, respectively.
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