研究目的
Investigating the electrochemical immunosensing of human IgE using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with Fc-modified SAMs on ITO electrodes.
研究成果
The study successfully developed an electrochemical immunosensor for human IgE detection using Fc-modified SAMs on ITO electrodes. The sensor demonstrated good sensitivity and selectivity, with a LOQ of 10 IU/mL, suitable for allergy diagnosis based on total IgE levels.
研究不足
The study mentions that the EIS method was less reproducible than CV measurements due to non-specific binding affecting charge transfer resistance. Pre-treatments such as filtration or separation are required for application in real serum.
1：Experimental Design and Method Selection
The study employed cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) for the detection of human IgE. Fc-modified SAMs were constructed on ITO electrodes to facilitate electron transfer and signal amplification.
2：Sample Selection and Data Sources
Human IgE serum samples were used as targets. The samples were prepared using PBS buffer, and various concentrations of target human IgE from 0 IU/mL to 300 IU/mL were tested.
3：List of Experimental Equipment and Materials
ITO electrodes, (3-Aminopropyl)triethoxysilane (APTES), ferrocenecarboxaldehyde, sodium borohydride (NaBH4), streptavidin, biotin-conjugated rabbit anti-human IgE antibody, ALP-conjugated goat anti-human IgE antibody, and other chemicals were used.
4：Experimental Procedures and Operational Workflow
The ITO electrodes were cleaned and modified with APTES SAMs, followed by Fc modification. The electrodes were then incubated with streptavidin, biotin-conjugated receptor antibody, target human IgE, and ALP-conjugated secondary antibody. Electrochemical measurements were performed after enzymatic reaction.
5：Data Analysis Methods
The data were analyzed using CV and EIS. The LOD, LOQ, and dynamic range were determined from the calibration curves.
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