研究目的
To screen and detect the harmful substance melamine (MEL) using a quantum-dot-bead-based immunochromatographic assay (QB-ICA) for rapid, sensitive, and quantitative onsite detection.
研究成果
The QB-ICA system demonstrated high sensitivity and practicability for MEL detection, offering a rapid and quantitative alternative to traditional methods. Its performance was comparable to ELISA but with the advantage of onsite applicability. The strategy holds promise for dynamic monitoring of food safety.
研究不足
The study focused on MEL detection in pure milk, and the applicability to other matrices was not explored. The pretreatment step, while improving sensitivity, adds complexity to the procedure.
1：Experimental Design and Method Selection:
The QB-ICA was formulated for MEL detection, optimizing parameters for sensitivity and specificity.
2：Sample Selection and Data Sources:
Pure milk samples spiked with MEL were used, with and without pretreatment.
3：List of Experimental Equipment and Materials:
Included QB with specific excitation and emission wavelengths, anti-MEL monoclonal antibody, and various buffers and reagents.
4：Experimental Procedures and Operational Workflow:
Involved the preparation of QB-mAbs, fabrication of the QB-ICA system, and the test procedure for MEL detection.
5：Data Analysis Methods:
The FIT/FIC ratio was used for establishing the calibration curve and calculating MEL concentrations.
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