研究目的
To examine the effect of conditioned media (CM) derived from BLED (5 J/cm2)-exposed human normal fibroblasts (CMBL5) for clinical application, focusing on its anti-inflammatory and cytoprotective effects.
研究成果
The conditioned media from normal cells exposed to BLED have anti-inflammatory and cytoprotective effects. BLED irradiation induced transient ER stress and increased expression of ER stress proteins in CCD-1131Sk cells. Treatment with CMBL5-LO protected from extracellular stimuli by maintaining membrane integrity, inhibiting apoptotic cell death and inflammatory mediators.
研究不足
The study did not fully clarify the molecular action of CMBL5-LO for anti-inflammatory activity. Further studies are needed to examine the precise mechanism by CMBL5-LO in cells.
1：Experimental Design and Method Selection:
The study used a BLED-irradiating device with 450-nm wavelength at a power of
2：0 mW/cmConditioned media (CMBL5) was collected from BLED-exposed human normal fibroblasts and concentrated using a centrifugal filter unit. The anti-inflammatory effect of CMBL5-LO was evaluated in LPS-stimulated RAW7 cells. Sample Selection and Data Sources:
Human fibroblast CCD-1131Sk and mouse macrophage RAW
3：7 cells were used. List of Experimental Equipment and Materials:
2 BLED-irradiating device, centrifugal filter units, hybrid tandem mass spectrometry for metabolomic profiling.
4：Experimental Procedures and Operational Workflow:
Cells were irradiated with BLED, conditioned media was collected and concentrated, and its effects on apoptosis and inflammation were assessed.
5：Data Analysis Methods:
Flow cytometry for apoptosis, cytokine array analysis for inflammatory cytokines, and hybrid tandem mass spectrometry for metabolomic profiling.
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