研究目的
To propose a photoacoustic (PA) method for noninvasively detecting blood viscosity in subcutaneous microvasculature with the frequency-resolved measurement.
研究成果
The frequency-resolved PA measurement can simultaneously provide the absorption and viscosity information of different vascular branches, demonstrating its potential for real-time monitoring of viscosity changes in subcutaneous microvasculature and its application in diagnosis and treatment.
研究不足
The method is limited by the size of the microvascular structures and the response characteristic of the transducer. The accuracy is affected by the spectrum analysis precision and the repetition frequency of the laser.
1：Experimental Design and Method Selection:
The study utilized a frequency-resolved photoacoustic measurement to characterize blood viscosity in subcutaneous microvasculature. The influence of viscosity on PA generation was investigated theoretically.
2：Sample Selection and Data Sources:
Water mixed with different concentrations of glycerol was measured to test the method's feasibility and accuracy. In vivo experiments were conducted on a BALB/c mouse's ear to monitor metabolism-induced viscosity changes.
3：List of Experimental Equipment and Materials:
A solid-state laser operating at 532 nm, a 10x microscope objective, a customized ultrasonic transducer with a center frequency of 75 MHz, and a digital oscilloscope were used.
4：Experimental Procedures and Operational Workflow:
The laser was focused on the target to generate PA signals, which were captured by the ultrasonic transducer, amplified, and recorded. Data were analyzed with fast Fourier transform by MATLAB.
5：Data Analysis Methods:
The FWHM of the PA frequency spectrum was obtained to characterize the viscosity of the sample.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
