研究目的
To investigate a novel imaging technique to identify the continuous posttrabecular aqueous outflow pathway from a single B-scan, using swept-source optical coherence tomography (SS-OCT).
研究成果
Continuous posttrabecular aqueous outflow pathway could be identified from a single B-scan, using SS-OCT with en face imaging and reslicing 3D volume data. This technique effectively reconstructs the complex 3D structure into visually apparent 2D images and promises to provide an opportunity for research in unexplored fields of the posttrabecular aqueous outflow pathway and the changes related to glaucoma surgery.
研究不足
1. The vessels might not have been perfectly delineated, especially in deeper locations. 2. The lateral resolution of the image was unknown due to the use of DRI OCT-1, originally designed for fundus imaging. 3. Discrimination between veins and arteries can be difficult. 4. Vessel images of the vasculature map were slightly faint. 5. The findings with the proposed method have not been validated in this study.
1:Experimental Design and Method Selection:
The study used swept-source optical coherence tomography (SS-OCT) to acquire three-dimensional volume scans of the anterior segment area at the limbus from healthy subjects. The aqueous outflow pathway was identified using an en face OCT image and reconstructed images of the vasculature.
2:Sample Selection and Data Sources:
Eleven eyes of 11 healthy subjects were included. The right eye from each subject was scanned.
3:List of Experimental Equipment and Materials:
A swept-source optical coherence tomography (SS-OCT) system (DRI OCT-1 model; Topcon, Tokyo, Japan) was used.
4:Experimental Procedures and Operational Workflow:
Subjects were instructed to gaze to the right or left side to scan the temporal or nasal side of the anterior segment area. The OCT probe was moved closer to the eye for image acquisition.
5:Data Analysis Methods:
The aqueous outflow pathway was identified using an en face OCT image and reconstructed images of the vasculature. The longitudinal diameter of the vessel was measured at a superficial location and the deepest location for analyses.
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