研究目的
To develop paper-based microchip electrophoresis (pME) devices for the separation of clinically relevant compounds, specifically BSA and creatinine, for the diagnosis of renal disorders.
研究成果
The pME-C4D device developed in this study is a cost-effective, lightweight, and robust platform for the rapid separation of clinically relevant compounds, offering a promising alternative for the diagnosis of renal disorders.
研究不足
The study focuses on the separation of BSA and creatinine, and while the device shows promise for clinical diagnosis, further studies are needed to validate its use with a wider range of biomolecules and in real clinical settings.
1：Experimental Design and Method Selection
The pME devices were fabricated using laser cut and thermal lamination processes with polyester pouches. Hand-drawn pencil electrodes were integrated for C4D.
2：Sample Selection and Data Sources
BSA and creatinine were used as the analytes of interest due to their clinical relevance in diagnosing renal diseases.
3：List of Experimental Equipment and Materials
Chromatographic paper, polyester thermal laminating pouches, paper punch, micropipette tips, stylet, epoxy glue, thermal laminator machine, CO2 laser engraver, office paper sheet, pencil grade 2B, adhesive tape, commercial or lab-made C4D system, function generator, bipolar high-voltage sequencer, background electrolyte (BGE), biomolecule stock solutions, artificial serum samples.
4：Experimental Procedures and Operational Workflow
The fabrication process involved designing the layout, cutting paper channels with a CO2 laser, laminating the paper microchip, creating reservoirs with micropipette tips, and integrating hand-drawn pencil electrodes. The electrophoresis and C4D detection procedure included preparing BGE and sample solutions, conditioning the channels, performing electrokinetic injections, and analyzing the samples.
5：Data Analysis Methods
The separation of BSA and creatinine was monitored using C4D, and the results were analyzed to evaluate the device's performance.
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