研究目的
To investigate formation of genotoxic byproducts during different UV-based treatments and identify the byproducts responsible for genotoxicity.
研究成果
MP UV can induce mutagenicity, while LP UV does not. H2O2 oxidation can degrade some genotoxic compounds, and BAC is more effective than chlorination in removing genotoxicity. Most mutagenicity was found in TA100 without S9, suggesting guanosine and/or cytosine adducts contribute to mutation. N-nitrosamines and histidine were excluded as genotoxic candidates, and 24 nitrogenous organic compounds were identified as potential genotoxic byproducts.
研究不足
The study was limited to specific UV-based treatment processes and may not cover all potential byproducts or conditions. The identification of genotoxic byproducts was tentative due to lack of reference standards and MS/MS spectra.
1：Experimental Design and Method Selection:
The study used Ames fluctuation test with Salmonella strains TA98 and TA100 to assess genotoxicity. LC-QTOF-MS was used for byproduct identification.
2：Sample Selection and Data Sources:
Water samples from pilot-scale studies treating secondary effluents in Tucson, Arizona, were used.
3：List of Experimental Equipment and Materials:
Included MP and LP UV lamps, H2O2, chlorine, BAC, and various chemicals for sample preparation and analysis.
4：Experimental Procedures and Operational Workflow:
Samples were treated with different UV-based processes, extracted, and analyzed for genotoxicity and byproducts.
5：Data Analysis Methods:
Genotoxicity data were analyzed using fold change over baseline, and byproducts were identified using LC-QTOF-MS with MassHunter software.
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