研究目的
Developing a novel fast response fluorogenic probe for monitoring hypochlorite (ClO?) in living cells and Zebrafishes, capable of detecting ClO? in extremely cold conditions and in vitro samples like urine.
研究成果
The probe D1 is a highly selective and fast-response fluorogenic probe for ClO?, capable of detecting ClO? in extremely cold conditions and in complex biological environments, making it suitable for disease diagnosing and monitoring in extreme conditions.
研究不足
The study does not address the probe's performance in detecting ClO? in other biological samples beyond HeLa cells and Zebrafishes, or its long-term stability and toxicity in vivo.
1：Experimental Design and Method Selection:
The probe D1 was developed based on the specific ClO? removal of a C=N bond, utilizing a naphthol fluorophore for its excellent water solubility properties.
2：Sample Selection and Data Sources:
HeLa cells and Zebrafishes were used to assess the probe's ability to monitor exogenous and endogenous ClO? levels.
3：List of Experimental Equipment and Materials:
Included a BioTek Cytatio5 Cell Imaging Multi-Mode Reader for fluorescence spectra and Zeiss LSM880 NLO Confocal Microscope System for imaging.
4：Experimental Procedures and Operational Workflow:
Cells and Zebrafishes were incubated with D1 under various conditions to monitor ClO? levels, with fluorescence intensity measured over time.
5：Data Analysis Methods:
Fluorescence intensity changes were analyzed to determine ClO? concentration, with selectivity and sensitivity assessed against various ROS/RNS and other biological analytes.
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