研究目的
Investigating the synthesis and optimization of silver nanoparticles (AgNPs) using whole-cell aqueous extract (WCAE) of green algae Neochloris oleoabundans under different conditions including light/dark conditions, AgNO3 concentration, pH, and extraction time.
研究成果
The WCAE of Neochloris oleoabundans showed the ability to mediate the reduction of Ag+ to fabricate AgNPs in the presence of light. The synthesized AgNPs were quasi-spherical with antibacterial activity on E. coli. The optimal conditions for AgNP biosynthesis were determined to be 0.8 mM AgNO3, pH 5, and 9-h extraction at a light intensity of 5000 lx.
研究不足
The study was limited by the low concentration of cellular materials in the WCAE, which seemed to be beyond the threshold of detection of corresponding analytic methods. Additionally, the inhibitory effects of low pH on AgNP synthesis and the adaptation of E. coli to AgNPs were noted as potential areas for optimization.
1：Experimental Design and Method Selection:
The study involved the synthesis of AgNPs by incubating the mixture of AgNO3 solution and WCAEs of Neochloris oleoabundans under light conditions. Single-factor and multi-factor optimization were conducted to evaluate the effects of AgNO3 concentration, pH, and extraction time.
2：Sample Selection and Data Sources:
Neochloris oleoabundans was cultivated in Modified Bristol Medium (MBM) until the microalgal cell density reached approximately 1.6 × 107 cells/mL. The culture was then centrifuged and rinsed with deionized water to produce medium free cell aqueous suspensions.
3：6 × 107 cells/mL. The culture was then centrifuged and rinsed with deionized water to produce medium free cell aqueous suspensions.
List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment included an illuminated incubator, centrifuge, ULT freezer, UV-VIS Spectrophotometer, TEM, and phase-contrast microscope. Materials included AgNO3, MBM components, and Neochloris oleoabundans.
4：Experimental Procedures and Operational Workflow:
The microalgal culture was thawed, incubated in a boiling water bath, centrifuged, and the supernatant (WCAE) was used for AgNP synthesis. The mixture of WCAE and AgNO3 was incubated under light or dark conditions.
5：Data Analysis Methods:
Spectrometric measurements were carried out using a UV-VIS Spectrophotometer. The morphology of AgNPs was observed by TEM. Antibacterial activity was tested by measuring the growth of E. coli in the presence of AgNPs.
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