研究目的
Evaluation of ochratoxin A (OTA) in coffee using compact surface plasmon resonance (SPR) biosensors based on crosslinked chitosan and carboxymethyl chitosan nanomatrix substrates.
研究成果
A rapid and sensitive competitive inhibition SPR immunoassay was developed on a 3D printed compact SPR system for the detection of OTA in coffee. The system demonstrated high sensitivity and cost-effectiveness, with potential applications at farm and industrial levels for OTA detection in food commodities.
研究不足
The study mentions that the developed SPR system was tested in ambient conditions, which might influence the properties of the polymer upon exposure and consequently affect the sensitivity of the system. Additionally, the complexity of coffee matrix may result in nonspecific binding of the sensor substrate.
1：Experimental Design and Method Selection:
The study utilized a compact SPR system designed based on edge coupling approach for the detection of OTA in coffee. The system involved the use of chitosan and carboxymethyl chitosan nanomatrix substrates for immobilization of OTA-BSA conjugate.
2：Sample Selection and Data Sources:
Coffee samples spiked with various concentrations of OTA were used. The samples were prepared by blending roasted coffee beans and spiking them with OTA.
3：List of Experimental Equipment and Materials:
The SPR system included a gold (Au) sensor chip, chitosan and carboxymethyl chitosan solutions, OTA-BSA conjugate, monoclonal ochratoxin A antibodies (mAb-OTA), and a microfluidic flow channel fabricated using polydimethylsiloxane (PDMS).
4：Experimental Procedures and Operational Workflow:
The SPR chips were coated with chitosan and carboxymethyl chitosan through spin coating technique. The OTA-BSA conjugate was immobilized on the sensor surface, and competitive inhibition immunoassay was developed for OTA detection.
5：Data Analysis Methods:
The optical spectra were recorded using a spectrometer, and data were processed with Origin lab 8. Logistic fit function was used for the calibration curve.
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