研究目的
Investigating the feasibility of direct imaging of adsorption of individual colloidal quantum dots in nanopores of a nuclear filter using fluorescence nanoscopy.
研究成果
The feasibility of direct imaging of ultra? ltration of semiconductor nanocrystals, functionalized by ligand shells, from a colloidal toluene solution in nanopores of a track membrane, using super-resolved ? uorescence spectroscopy, has been demonstrated. Fluorescent images of individual nanocrystals (quantum dots) and their agglomerates were recorded at different depths along the thickness of a track membrane, after ultra? ltration and complete evaporation of the solvent.
研究不足
The technique is limited by the resolution of the optical microscope and the signal-to-noise ratio of the detected luminescence. The axial spatial coordinate cannot be determined to high accuracy from analysis of the diffraction image for a single point source.
1：Experimental Design and Method Selection:
Fluorescence nanoscopy was used for direct imaging of adsorption of individual colloidal quantum dots in nanopores.
2：Sample Selection and Data Sources:
Colloidal quantum dots (CdSeS/ZnS, diameter ~6 nm) and polypropylene track membranes with pores of diameter ~500 nm were used.
3：List of Experimental Equipment and Materials:
Melles Griot 40 ×
4：6NA microscope objective, piezoelectric motion-controlled translator with EG100 Stage Controller, single-frequency continuous-wave Coherent Verdi V6 laser, dichroic mirror/beamsplitter (Thorlabs DMLP605), interference filters (Thorlabs FELH 600, Semrock SR628/32), ultrasensitive cooled electron multiplying CCD camera (Andor iXon Ultra EMCCD). Experimental Procedures and Operational Workflow:
A colloidal solution of quantum dots was passed through a propylene membrane, and the process was imaged at different focal depths.
5：Data Analysis Methods:
Data was processed using special software based on an original image recognition algorithm, including preliminary ? ltering of the noise background, searching for local intensity maxima, and approximation of the localized images using the Levenberg–Marquardt algorithm.
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CCD camera
iXon Ultra EMCCD
Andor
Recording luminescent images of the individual quantum dots and their agglomerates.
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Laser
Coherent Verdi V6
Coherent
Excitation of luminescence in the quantum dots.
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Dichroic mirror/beamsplitter
DMLP605
Thorlabs
Spectral separation of the exciting radiation and the detected radiation.
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CdSeS/ZnS quantum dots
Sigma Aldrich
Fluorescent markers for imaging adsorption in nanopores.
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Melles Griot microscope objective
40 × 0.6NA
Melles Griot
Used for collecting luminescent emission from the quantum dots.
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Piezo-scanning translator
EG100 Stage Controller
Nano Scan Technology
Precision displacement of the microscope objective.
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Interference filters
FELH 600, SR628/32
Thorlabs, Semrock
Separation of the ? uorescent emission from the scattered laser emission.
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