研究目的
To investigate the functional behavior of E171 and P25 TiO2 nanoparticles in solutions of bovine serum albumin (BSA) and sucrose as model food ingredients, and to understand the suitability of using P25 as a substitute for E171 in toxicity tests for food-related risk assessment.
研究成果
The study demonstrated significant differences in the behavior of P25 and E171 TiO2 nanoparticles in food ingredient solutions, highlighting the importance of using food-grade E171 for accurate toxicological assessments. P25 should not be used as a substitute for E171 in food-related risk assessments due to underestimation of dosage and potential toxicity.
研究不足
The study focused on model food ingredients (BSA and sucrose) and may not fully represent the complexity of real food matrices. The ISDD model assumes idealized conditions that may not capture all in vivo dynamics.
1：Experimental Design and Method Selection
The study compared the behavior of P25 and E171 TiO2 nanoparticles in model food ingredient solutions (BSA and sucrose) to understand their interaction and potential toxicological impacts. Methods included dynamic light scattering (DLS), electrophoretic light scattering (ELS), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and in vitro sedimentation, diffusion and dosimetry (ISDD) modeling.
2：Sample Selection and Data Sources
P25 and E171 TiO2 nanoparticles were used as test materials. BSA and sucrose were chosen as model food ingredients. Data were collected on hydrodynamic size, surface charge, protein and sucrose corona formation, and sedimentation profiles.
3：List of Experimental Equipment and Materials
ZetaSizer Nano-ZS (Malvern Instruments, UK) for DLS and ELS; FTIR (Perkin Elmer Spectrum GX, USA); TEM (Carl Zeiss Libra 120); TGA (TA Instruments Q500, New Castle); MATLAB for ISDD simulations.
4：Experimental Procedures and Operational Workflow
NPs were dispersed in BSA and sucrose solutions at various concentrations. Hydrodynamic size and zeta potential were measured. Protein and sucrose coronas were analyzed using FTIR, TEM, and TGA. Sedimentation profiles were simulated using ISDD.
5：Data Analysis Methods
Statistical analyses were performed using Student's t-test or one-way ANOVA with Tukey's post-hoc testing. Significance was accepted at p < 0.05.
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