研究目的
To enhance periphytic biofilm performance in nitrogen removal via upconversion luminescence of upconversion phosphors (UCPs) and investigate the microbial community composition and structure, and carbon source utilization capacity of the periphytic biofilm stimulated by UCPs.
研究成果
The study demonstrates that stimulation by UCPs doped with Pr3+ and Li+ significantly enhances nitrogen removal by periphytic biofilms from both high and low nitrogen concentration waters. The optimized microbial community structure and improved carbon source metabolic capacity contribute to the enhanced nitrogen removal efficiency. This novel approach shows promise for application in wastewater and surface water treatment systems with fluctuating nitrogen concentrations.
研究不足
The study focuses on the effects of UCPs on periphytic biofilms for nitrogen removal, but the long-term stability and scalability of this approach in real-world applications are not addressed. The specific mechanisms by which UCPs optimize microbial community structure and enhance nitrogen removal require further investigation.
1：Experimental Design and Method Selection:
The study employed a bench-scale simulation of a periphytic biofilm wastewater treatment system to examine the efficiency of nitrogen removal by periphytic biofilm, investigate the microbial community composition and structure, and determine the carbon source utilization capacity of the periphytic biofilm stimulated by UCPs coped with Pr3+ and Li+.
2：Sample Selection and Data Sources:
Periphytic biofilms were cultured from water collected from Xuanwu Lake, Nanjing, China. High and low nitrogen concentration experiments were conducted to evaluate nitrogen removal efficiency.
3：List of Experimental Equipment and Materials:
Fiber carriers for periphytic biofilm substrates, UCPs doped with Pr3+ and Li+, X-ray diffractometer, three-dimensional fluorescence spectrometer, transmission electron microscope, scanning electron microscopy, energy dispersive spectrometry, Biolog E coplatesTM, flow injection analyzer.
4：Experimental Procedures and Operational Workflow:
Periphytic biofilms were activated with UCPs, followed by nitrogen removal experiments in simulated wastewater and surface water systems. Microbial community structures and carbon source metabolic capacities were analyzed.
5：Data Analysis Methods:
Principal Component Analysis (PCA) and Canonical Analysis (CA) were used for carbon source utilization and fatty acids data. One-way ANOVA was used for statistical analysis.
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