研究目的
To investigate changes in human stratum corneum using reflectance NIRS during acute barrier disruption and to provide a non-invasive method to monitor stratum corneum based on molecular changes in protein, lipid on SC.
研究成果
Reflectance NIRS can be used to monitor barrier function in stratum corneum based on changes in protein and lipid. It provides a non-invasive method to monitor changes in protein, lipid and water in stratum corneum, which can be useful for investigating skin disorders related with barrier disruption.
研究不足
The study was conducted on a limited number of healthy volunteers without dermatological disorders. Further studies are required to investigate skin with disorders such as atopic dermatitis using NIRS.
1：Experimental Design and Method Selection:
Reflectance NIRS was used to investigate the effect of disruption of barrier on spectra of skin. Multivariate statistical analysis and second derivative analysis of human skin spectra were performed.
2：Sample Selection and Data Sources:
Sixteen healthy volunteers without any dermatological disorder participated. NIR skin spectra were collected using VERTEX 80 FTIR spectrometer.
3：List of Experimental Equipment and Materials:
VERTEX 80 FTIR spectrometer (Bruker, Germany), fiber optic probe (Bruker, Germany), 3M scotch tape, Dermalab Combo (Cortex Technology, Denmark), Spectralon (Labsphere, New Hampshire).
4：Experimental Procedures and Operational Workflow:
Site for analysis was marked and 3M scotch tape was used for tape stripping of stratum corneum in volar forearm to induce acute barrier disruption. Before tape stripping, TEWL was measured and NIR spectra were collected for baseline measurement. After each three consecutive tape stripping with adhesive tape on volar forearm, TEWL was measured and skin spectra were obtained.
5：Data Analysis Methods:
Standard Normal Variate (SNV) method was applied for the pre-treatment of spectra obtained from each subject. Principal component analysis (PCA) was performed for multivariate analysis of skin spectra. The second derivative of skin spectra were calculated with the Savitzky-Golay derivative.
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VERTEX 80 FTIR spectrometer
VERTEX 80
Bruker
Used to collect NIR skin spectra in the spectral region of 7000 cm-1 to 4000 cm-1 at 16 cm-1 resolution with an InGaAs detector.
VERTEX 80 & 80v FT-IR Spectrometers
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fiber optic probe
Bruker
Used to obtain reflectance spectrum on human skin, contains bundle of optical fiber with illumination fiber and detecting fiber.
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Dermalab Combo
Cortex Technology
Used to measure TEWL (Trans Epidermal Water Loss).
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Spectralon
Labsphere
Used to obtain reference spectrum to calculate absorbance.
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