研究目的
To demonstrate that synchrotron radiation phase-contrast imaging (SR-PCI) can be used to visualize the intrascalar structures in implanted human cochleae and to find the optimal combination of the parameters object-to-detector distance (ODD) and beam energy (E) for visualization.
研究成果
SR-PCI is a viable method to visualize implanted human cochleae, with the optimal combination of E and ODD parameters being 72 keV and 2 m, respectively. This method is less invasive, less labour intensive, and associated with a much lower acquisition time compared to other methods for postimplantation imaging in humans.
研究不足
1. There was a time gap between the preparation, implantation and scanning, leading to potential degradation of intracochlear membranes. 2. Only five combinations of ODD and E were examined due to time constraints. 3. The results depended on which voxels were sampled and the geometric configuration of the specimen, potentially introducing bias.
1：Experimental Design and Method Selection:
SR-PCI was used with varying combinations of parameters ODD (3, 2 and 1 m) and E (47, 60 and 72 keV) to image three cadaveric implanted human temporal bones.
2：Sample Selection and Data Sources:
Three temporal bones from two freshly frozen cadaveric human heads were used.
3：List of Experimental Equipment and Materials:
Bio-Medical Imaging and Therapy beamline at the Canadian Light Source Inc., Matlab (Version 2017b, MathWorks Inc.), Flex28 CI electrode (MED-EL, Innsbruck, Austria).
4：Experimental Procedures and Operational Workflow:
Samples were implanted with a CI electrode, fixed, and then scanned with SR-PCI at different ODD and E combinations. Images were reconstructed to a 3D stack of slices.
5：Data Analysis Methods:
The acquired 3D images were compared using CNRs of the basilar membrane and the electrode array and the standard deviation of the beam streaks. Postprocessing calculations were performed using Matlab.
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