摘要： Hyperpolarized MRI with 13C‐labeled metabolites has enabled metabolic imaging of tumors in vivo. The heterogeneous nature of tumors and the limited lifetime of the hyper-polarization require high resolution, both temporally and spatially. We describe two sequences that make more efficient use of the 13C polarization than previously described single‐shot 3D sequences. With these sequences, the target metabolite resonances were excited using spectral‐spatial pulses and the data acquired using spiral readouts from a series of echoes created using a fast‐spin‐echo sequence employing adiabatic 180° pulses. The third dimension was encoded with blipped gradients applied in an interleaved order to the echo train. Adiabatic inversion pulses applied in the absence of slice selection gradients allowed acquisition of signal from odd echoes, formed by unpaired adiabatic pulses, as well as from even echoes. The sequences were tested on tumor‐bearing mice following intra-venous injection of hyperpolarized [1‐13C]pyruvate. [1‐13C] pyruvate and [1‐13C] lactate images were acquired in vivo with a 4 × 4 × 2 cm3 field of view and a 32 × 32 × 16 matrix, leading to a nominal resolution of 1.25 × 1.25 × 1.25 mm3 and an effective resolution of 1.25 × 1.25 × 4.5 mm3 when the z‐direction point spread function was taken into account. The acquisition of signal from more echoes also allowed for an improvement in the signal‐to‐noise ratio for relaxation times. The pulse sequences described here produced hyperpolarized 13C images with improved resolution and signal‐to‐noise ratio when compared with similar sequences described previously.