研究目的
To develop a new selective and reusable sensor for the fluorescent and colorimetric detection of cyanides, utilizing a novel CH-group activation strategy to construct a FRET platform.
研究成果
The study successfully developed a new selective and reusable sensor for the fluorescent and colorimetric detection of cyanides, utilizing a novel CH-group activation strategy to construct a FRET platform. The sensor exhibits clear and significant optical changes in solutions as well as inside living cells, suggesting its potential for future development of fluorophore scaffolds for anion sensing.
研究不足
The study focuses on the detection of cyanides and does not explore the sensor's response to a wider range of anions or under varying environmental conditions. The practical utility in real-world applications, such as environmental monitoring, requires further investigation.
1：Experimental Design and Method Selection:
The study involved the synthesis of a fluorescent and colorimetric cyanide sensor (4-Br) based on the activated C-H group to construct a FRET platform. The sensing mechanism integrates ICT and FRET mechanisms.
2：Sample Selection and Data Sources:
The sensor's response to cyanides and other anions was evaluated in CH3CN/H2O solution.
3：List of Experimental Equipment and Materials:
The synthesis involved control compounds 1, 2, and 4, and the target probe 4-Br was prepared through a series of chemical reactions.
4：Experimental Procedures and Operational Workflow:
The sensor's chromogenic and fluorescent behaviors were assessed upon addition of TBACN and other anions. Time-dependent studies and fluorescence imaging in living cells were conducted.
5：Data Analysis Methods:
UV-visible and fluorescence spectra were recorded to analyze the sensor's response to cyanides. The detection limit was calculated, and the sensor's applicability in living cells was examined.
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