研究目的
To explore the use of silver nanoparticles and NIR laser excitation for SERS applications for biochemical analysis and detection in human liver tissue, aiming for early HCC detection.
研究成果
The study demonstrates that Ag colloid based SERS spectroscopy can differentiate HCC, adjacent HCC tissues from normal control tissues with high diagnostic sensitivity and specificity, suggesting a promising tool for early HCC screening.
研究不足
The study suggests further increasing the number of samples and building a Raman database of liver tissues from HCC patients in different stages for more effective application in clinic.
1：Experimental Design and Method Selection:
SERS was employed to obtain HCC tissue spectra with coated silver nanoparticles. PCA combined with LDA was used for spectra analysis.
2：Sample Selection and Data Sources:
Liver tissue samples from 48 patients (23 normal, 48 adjacent cancer and 48 cancerous tissues) were provided by The First Affiliated Hospital of Fujian Medical University.
3：List of Experimental Equipment and Materials:
Colloidal Ag nanoparticles prepared by the aqueous reduction of silver nitrate with hydroxylamine hydrochloride, a confocal microscope Raman system (Renishaw, Great Britain), and a 785 nm diode laser.
4：Experimental Procedures and Operational Workflow:
Tissue sections were coated with Ag colloidal solution, dried, and then SERS spectra were obtained. Spectra were collected over a wave-number range of 400–1800 cm?1 with an integration time of 10 s.
5：Data Analysis Methods:
Multivariate data analysis including PCA, LDA, and ROC curve was executed on the measured SERS spectra processed by SPSS 15.0 software.
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