研究目的
To develop a fluorometric assay for hydroquinone (HQ) using N/S/P-codoped carbon dots (CDs) as a fluorescent probe, based on the formation of ground state complexes between CD and Fe(III) leading to fluorescence quenching and recovery upon addition of HQ.
研究成果
The N/S/P-codoped CDs-Fe3+ system was successfully applied as a turn-off-on fluorescence approach for HQ detection, showing high specificity and sensitivity. The method was effective in complex matrices like water and developer samples.
研究不足
The excitation wavelength is in the UV region, making the probe prone to interference by biomatter. The system may also be affected by ascorbic acid (AA) or hydrogen peroxide (H2O2).
1：Experimental Design and Method Selection
The study utilized a one-step hydrothermal reaction for the preparation of N/S/P-codoped CDs from glyoxal and isocarbophos. The method is based on the fluorescence quenching of CDs by Fe(III) and its recovery upon the oxidation of HQ by Fe(III).
2：Sample Selection and Data Sources
The samples included spiked water and developer samples for the determination of HQ. The fluorescence spectra were recorded in the presence and absence of HQ.
3：List of Experimental Equipment and Materials
Glyoxal, isocarbophos, HQ, and various metal salts were used. The equipment included a Teflon-sealed autoclave reactor, F-7000 spectrophotometer, UV-2550 ultraviolet spectrophotometer, JEM 2100 electron microscope, Nicolet 6700 FT-IR spectrometer, K-Alpha XPS spectrometer, and Coolpix 4500 digital camera.
4：Experimental Procedures and Operational Workflow
The CDs were synthesized via hydrothermal heating. The detection procedure involved mixing CD dispersion with Fe3+ solution, followed by the addition of HQ solutions of different concentrations. Fluorescence spectra were recorded at an excitation wavelength of 363 nm.
5：Data Analysis Methods
The fluorescence intensity changes were analyzed to quantify HQ concentration, with a linear relationship established between fluorescence recovery and HQ concentration.
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Teflon-sealed autoclave reactor
DZ-2A
Tianjin Taisite Instrument Co., Ltd.
Used for the synthesis of N/S/P-codoped CDs via hydrothermal reaction.
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F-7000 spectrophotometer
Hitachi
Used for recording fluorescence spectra and intensity.
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UV-2550 ultraviolet spectrophotometer
Shimadzu
Used for absorption spectral measurements.
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JEM 2100 electron microscope
JEOL Ltd.
Used for TEM data acquisition.
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Nicolet 6700 FT-IR spectrometer
Thermo Scientific Ltd.
Used for FT-IR spectra acquisition.
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K-Alpha XPS spectrometer
Thermo Scientific Ltd.
Used for XPS measurements.
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Coolpix 4500 digital camera
Nikon
Used for capturing digital photos of CD solution under daylight and UV lamp illumination.
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