研究目的
To establish a sensitive, non-destructive, time saving, and label-free method for MC-LR identification to monitor water quality and safety worldwide.
研究成果
The study demonstrated that confocal Raman spectroscopy with label-free surface-enhanced Raman scattering strategy can effectively identify trace MC-LR, offering a potential method for monitoring water quality and safety worldwide.
研究不足
The identification of trace MC-LR was affected by impurities in realistic water samples, indicating the necessity for pretreatment and separation technologies.
1：Experimental Design and Method Selection:
Confocal Raman spectroscopy combined with SERS-AG substrate was used to characterize the 'fingerprint' information of MC-LR directly.
2：Sample Selection and Data Sources:
MC-LR and MC-RR standards were prepared in ultrapure water.
3：List of Experimental Equipment and Materials:
A Renishaw inVia micro-Raman spectroscopy system, SERS-AG substrates, and various concentrations of MC-LR and R6G solutions.
4：Experimental Procedures and Operational Workflow:
Samples were deposited on SERS-AG substrates, and Raman signals were collected with specific laser powers and objectives.
5：Data Analysis Methods:
An unsupervised exploratory principal component analysis (PCA) was used to assess the impacts of interferences on the SERS detection of MC-LR.
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