研究目的
The main objective of this work is to make a simple design that increase sensitivity of the absorbance spectrophotometer detector to detect the RNA sample without adding dyes or other flours material.
研究成果
In this work simplified double beam spectrophotometer is developed and successfully validated. Results on the test sample showed that the transimpedance amplifier and photodiodes can be used to build a simplified detector for the double beam spectrophotometer with excellent results. The peak present in 270nm clearly indicates the presence of RNA in the sample.
研究不足
The main challenges include high sensitivity, specificity, and protocols that conduct the analysis in a short time with low detection limit and low volume. Problems facing the performance of the spectrophotometer systems mainly come from the detector circuit such as dark current for the sensor and the oscillations caused by a capacitor diode form the photodiode on the input.
