研究目的
Investigating the reusability of capacitive field-effect electrolyte-insulator-semiconductor (EIS) sensors modified with a cationic weak polyelectrolyte for the label-free electrical detection of DNA.
研究成果
The study successfully demonstrated the reusability of PAH-modified capacitive field-effect EIS sensors for the label-free electrical detection of DNA. The sensors could be regenerated and reused for multiple DNA-detection measurements, with the signal changes indicating successful DNA immobilization or hybridization. The results were validated by fluorescence microscopy, confirming the feasibility of the approach for DNA biosensing applications.
研究不足
The study demonstrates the reusability of the sensors for at least five DNA-detection measurements, but the amplitude of the signal changes tends to decrease with increasing number of macromolecular layers. The efficiency of the sensor is also affected by the ionic strength of the measurement solution.
1：Experimental Design and Method Selection:
The study utilized capacitive field-effect electrolyte-insulator-semiconductor (EIS) sensors modified with a cationic weak polyelectrolyte (PAH) for the label-free electrical detection of DNA. The methodology involved the layer-by-layer electrostatic adsorption of PAH and DNA molecules onto the sensor surface.
2：Sample Selection and Data Sources:
Synthetic DNA oligonucleotides were used, including probe ssDNA and FAM-labeled ssDNA, complementary target cDNA and FAM-labeled cDNA. The DNA solutions were prepared in deionized water or phosphate buffered saline (PBS).
3：List of Experimental Equipment and Materials:
The equipment included EIS sensor chips, a Zennium impedance analyzer for electrochemical measurements, a fluorescence microscope for optical measurements, and various fluorescence dyes (FITC, FAM, SG).
4：Experimental Procedures and Operational Workflow:
The sensor chips were modified with PAH and DNA molecules through electrostatic adsorption. The ConCap method was used for electrochemical characterization. Fluorescence microscopy was employed to validate the surface modifications.
5：Data Analysis Methods:
The ConCap signal changes were analyzed to determine the effect of DNA immobilization and hybridization. Fluorescence intensity measurements were used to confirm the presence of DNA on the sensor surface.
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