研究目的
To develop a highly sensitive and selective porphyrin-based fluorescent sensor for the detection of thiophenol, leveraging the steric hindrance effect to differentiate thiophenol from biothiols.
研究成果
The porphyrin-based fluorescent sensor M1 demonstrates rapid, sensitive, and selective detection of thiophenol, with a low detection limit of 54 nM. The steric hindrance effect enhances the sensor's selectivity towards thiophenol over biothiols. This approach provides valuable insights for designing turn-on fluorescent sensors for other analytes.
研究不足
The study's limitations include the specific solvent system requirement (50% acetonitrile) for optimal fluorescence response and the potential interference from other species in complex biological samples.
1：Experimental Design and Method Selection:
The study involved the synthesis of a porphyrin-based fluorescent sensor (M1) with a 7-nitrobenzo[c][1,2,5]oxadiazolyl group linked to the aromatic ortho-position in the porphyrin to quench fluorescence. The sensor's response to thiophenol was evaluated based on fluorescence recovery.
2：Sample Selection and Data Sources:
Thiophenol and its derivatives were used as analytes. The sensor's response was tested against various anions and molecules to assess selectivity.
3：List of Experimental Equipment and Materials:
Instruments included a Shimadzu RF-5301 PC spectrophotometer for fluorescence spectra, a CT-6020A pH meter for pH evaluation, a 500 MHz NMR spectrometer (Bruker Avance) for 1H NMR spectra, and a Bruker Aupoflex-III Mass spectrometer for mass spectrometry.
4：Experimental Procedures and Operational Workflow:
The sensor M1 was synthesized and characterized. Fluorescence measurements were conducted in PBS buffered water solution with acetonitrile as a cosolvent. The sensor's response to thiophenol was measured over time and at varying concentrations.
5：Data Analysis Methods:
Fluorescence intensity ratios (F/F0) were plotted against thiophenol concentrations to establish a linear working curve. The detection limit was calculated based on S/N = 3.
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