研究目的
Investigating the fabrication and application of a simple, cheap, and functional surface enhanced Raman scattering substrate for biomedical applications, focusing on the protection of silver nanoparticles from oxidation and the enhancement of Raman signals through trapping bacteria.
研究成果
The study successfully fabricated a simple and functional SERS substrate with silver nanoparticles distributed in a three-dimensional porous membrane. The substrate demonstrated the ability to protect silver nanoparticles from oxidation and enhance Raman signals by trapping bacteria, indicating its potential for reliable biomedical applications.
研究不足
The study focuses on the fabrication and preliminary testing of the SERS substrate with a specific bacterium, Staphylococcus aureus. The long-term stability and effectiveness of the substrate with other types of bacteria or biomolecules were not explored.
1：Experimental Design and Method Selection:
The study involved the fabrication of a three-dimensional porous membrane decorated with silver nanoparticles to create hotspots for surface enhanced Raman scattering (SERS). The method included the preparation of Tollen's reagent and its reaction with glucose to deposit silver nanoparticles on the membrane.
2：Sample Selection and Data Sources:
A filter membrane was used as the substrate, and Staphylococcus aureus cells were used to test the biomedical application of the substrate.
3：List of Experimental Equipment and Materials:
Silver nitrate, glucose, ammonium hydroxide, filter membrane, scanning electron microscopy (SEM, Hitachi S3400), Advantage Near Infrared Raman Spectrometer.
4：Experimental Procedures and Operational Workflow:
The membrane was cleaned, Tollen's reagent was prepared and reacted with glucose on the membrane, and the membrane was then used to trap bacteria for Raman signal enhancement.
5：Data Analysis Methods:
SEM was used to investigate the morphology of the substrate, and Raman spectroscopy was used to measure the enhancement of Raman signals.
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