研究目的
Investigating the binding interactions of a synthesized carbazole ligand with human telomeric G-quadruplex DNA in Na+ and K+-containing buffer and its biological activity.
研究成果
The carbazole derivative 3 can stabilize and induce the formation of G-quadruplex structures in human telomeric DNA, showing higher cytotoxicity against breast cancer cells than non-tumorigenic breast epithelial cells, with its cytotoxic activity not directly associated with telomerase inhibition.
研究不足
The study did not achieve saturation of binding curves in both UV-Vis and fluorescence methods, making the estimation of K values using the Scatchard equation impossible. The binding product nKb from fluorescence data for the antiparallel 22HT G-quadruplex was almost half lower than that obtained from absorbance measurements.
1：Experimental Design and Method Selection:
The study employed UV-Vis spectrophotometry, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and DNA melting to investigate the interaction between the carbazole ligand and G-quadruplex DNA.
2：Sample Selection and Data Sources:
The human telomeric sequence 5′-AGGG(TTAGGG)3-3′ (22HT) was used.
3：List of Experimental Equipment and Materials:
A Cecil CE-2021 spectrophotometer, Jasco FP 8200 spectrofluorimeter, and Jasco J-810 spectropolarimeter were used.
4：Experimental Procedures and Operational Workflow:
The ligand was titrated with G4 DNA, and changes in absorption, fluorescence, and CD spectra were monitored.
5：Data Analysis Methods:
The Benesi–Hildebrand method was used for binding constant estimation, and Job plots were constructed for stoichiometry determination.
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