研究目的
To explore more efficient and less costly anticancer agents, various organotin derivatives have been investigated for their structures and anti-proliferative properties against a number of cell lines. The study focuses on the synthesis and characterization of two-photon absorption (2PA) organotin (IV) cyano carboxylate complexes for targeting nuclear with anticancer activities.
研究成果
The study successfully synthesized two novel organotin complexes (C1Sn-1 and C1Sn-2) with enhanced two-photon absorption properties. C1Sn-2 demonstrated significant potential as a two-photon anticancer agent targeting nuclear DNA with non-significant toxicity in animal models. This work offers a dual functional organotin complex for two-photon anticancer agent and nuclear targeting, which could have significant potentials in utilizing such complexes as biomedical candidates.
研究不足
The study is preliminary, and further research is needed to fully understand the mechanisms of action and potential side effects of the synthesized organotin (IV) complexes in vivo.
1：Experimental Design and Method Selection:
The study involved the synthesis and characterization of two novel 2PA organotin (IV) cyano carboxylate complexes (C1Sn-1, C1Sn-2). The nonlinear optical (NLO) studies were conducted to evaluate their two-photon absorption properties.
2：2). The nonlinear optical (NLO) studies were conducted to evaluate their two-photon absorption properties. Sample Selection and Data Sources:
2. Sample Selection and Data Sources: The anticancer activities of complexes C1Sn-1 and C1Sn-2 were evaluated against three tumor cell lines (HepG2, Hela, A549) and one normal cell HELF using methyl thiazolyl tetrazolium (MTT) assay.
3：List of Experimental Equipment and Materials:
The study utilized two-photon fluorescence microscopy (2PFM) for imaging, transmission electron microscopy (TEM) for localization studies, and flow cytometry for apoptosis analysis.
4：Experimental Procedures and Operational Workflow:
The intracellular distribution of C1Sn-2 was evaluated in A549 cells, and the anticancer effect was investigated in animal models.
5：Data Analysis Methods:
The data were analyzed using Gaussian and Discovery Studio Software for molecular modeling calculations, and the results were presented as means ± standard deviations (SD).
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