研究目的
To present an in-house designed stainless steel sublimator for matrix deposition in MALDI mass spectrometry imaging, enabling optimal matrix deposition reproducibility.
研究成果
The in-house designed stainless steel sublimator significantly improves matrix deposition reproducibility and reduces sample preparation time compared to standard glass sublimators, without compromising spatial resolution or signal intensity in MALDI-IMS experiments.
研究不足
The study focused on lipid analysis in human colon biopsies; applicability to other tissue types or analytes was not explored. The sublimator's performance with different matrices or under varying conditions was not fully investigated.
1：Experimental Design and Method Selection:
The study involved designing a stainless steel sublimator for matrix deposition in MALDI-IMS, comparing its performance with standard glass sublimators.
2：Sample Selection and Data Sources:
Human colon biopsies were used as samples.
3：List of Experimental Equipment and Materials:
Included a stainless steel sublimator prototype, glass sublimator, matrices (MBT and DAN), and MALDI-Orbitrap XL mass spectrometer.
4：Experimental Procedures and Operational Workflow:
Matrix deposition was performed using both sublimators under controlled conditions, followed by MALDI imaging mass spectrometry.
5：Data Analysis Methods:
Data were analyzed using Xcalibur software and MSIAnalyst for image visualization and analysis.
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