研究目的
Investigating the synthesis of TiO2(200)-rGO hybrid nanosheets for aptamer-based impedimetric determination of the prostate specific antigen (PSA).
研究成果
The study successfully synthesized rGO-TiO2 nano hybrids via a scalable and cheap hydrothermal method, demonstrating an effective synergism between GO and TiO2 for the immobilization of aptamer molecules. The aptasensor showed high sensitivity, selectivity, and stability, making it suitable for the detection of PSA and potentially other proteins and small molecules.
研究不足
The major limitation of the aptasensor is its relatively costly production.
1：Experimental Design and Method Selection:
The study involved the synthesis of TiO2(200)-rGO hybrid nanosheets via a scalable hydrothermal process and their characterization using SEM, FESEM-EDX, XRD, XPS, Raman, and FTIR spectroscopies.
2：Sample Selection and Data Sources:
The samples were synthesized from TiO2, rGO, and NaOH solid powders.
3：List of Experimental Equipment and Materials:
The study used a glassy carbon electrode (GCE), platinum counter electrode, Ag/AgCl reference electrode, and various chemicals including PSA binding DNA aptamer and PSA.
4：Experimental Procedures and Operational Workflow:
The modified GCE was prepared by drop casting nanocomposite dispersion on the cleaned GCE, followed by aptamer immobilization and PSA detection using electrochemical impedance spectroscopy (EIS) and voltammetry.
5：Data Analysis Methods:
The data were analyzed using NOVA 1.11 software for electrochemical measurements.
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