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A wavelength-dispersive instrument for characterizing fluorescence and scattering spectra of individual aerosol particles on a substrate

DOI:10.5194/amt-9-3987-2016 期刊:Atmospheric Measurement Techniques 出版年份:2016 更新时间:2025-09-04 15:30:14
摘要: We describe a novel, low-cost instrument to acquire both elastic and inelastic (fluorescent) scattering spectra from individual supermicron-size particles in a multi-particle collection on a microscope slide. The principle of the device is based on a slitless spectroscope that is often employed in astronomy to determine the spectra of individual stars in a star cluster but had not been applied to atmospheric particles. Under excitation, most commonly by either a 405 nm diode laser or a UV light-emitting diode (LED), fluorescence emission spectra of many individual particles can be determined simultaneously. The instrument can also acquire elastic scattering spectra from particles illuminated by a white-light source. The technique also provides the ability to detect and rapidly estimate the number fraction of fluorescent particles that could contaminate a collection of non-fluorescent material, even without analyzing full spectra. Advantages and disadvantages of using black-and-white cameras compared to color cameras are given. The primary motivation for this work has been to develop an inexpensive technique to characterize fluorescent biological aerosol particles, especially particles such as pollen and mold spores that can cause allergies. An example of an iPhone-enabled device is also shown as a means for collecting data on biological aerosols at lower cost or by utilizing citizen scientists for expanded data collection.
作者: Donald R. Huffman,Benjamin E. Swanson,J. Alex Huffman
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To develop a low-cost instrument capable of characterizing fluorescence and scattering spectra from individual aerosol particles on a substrate, with applications in detecting biological aerosols such as pollen and mold spores.

The developed instrument provides a cost-effective means to characterize fluorescence and elastic scattering spectra of individual aerosol particles. It enables rapid estimation of fluorescent particle fractions and has potential applications in bioaerosol detection, including pollen and mold spore monitoring. The smartphone version offers portability and the possibility for widespread data collection through citizen science.

The technique is limited by particle density on the slide to avoid spectral overlap. Spectral resolution is influenced by particle size and homogeneity. The method is currently optimized for supermicron-sized particles, though it can be adapted for smaller particles with higher magnification.

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