研究目的
To develop a label-free, high-throughput method for parallel, in vitro, and real-time analysis of specific single-cell signaling using hyperspectral photonic crystal resonant technology.
研究成果
The study presents a real-time and label-free single-cell signaling analysis method based on photonic crystal resonant surfaces. This method enables high-throughput measurements of the dynamics of protein secretion from single cells within a cell population, providing a simple yet powerful approach for studying complex physiological systems at single-cell resolution.
研究不足
The temporal resolution is limited by the instrumental limitations of the hyperspectral imaging setup, with a rate of 2.5 min per image. The specificity of the assay depends on the functionalization of the PCRS with specific antibodies.
1：Experimental Design and Method Selection:
The study employed hyperspectral photonic crystal resonant imaging to monitor protein secretion from single cells in real time.
2：Sample Selection and Data Sources:
BHK-TPO and HepG2 cell lines were used to study thrombopoietin (TPO) secretion. Human platelets with differing levels of desialylation were also used.
3：List of Experimental Equipment and Materials:
Photonic crystal resonant surfaces (PCRS), hyperspectral imaging setup, and specific surface functionalization towards TPO proteins.
4：Experimental Procedures and Operational Workflow:
Cells were cultured and exposed to the functionalized PCRS. Hyperspectral images were captured to monitor TPO secretion.
5：Data Analysis Methods:
The secretion of TPO was modeled as a Langmuir adsorption isotherm to quantify the kinetics of protein secretion.
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