研究目的
Developing perfluorocarbon-based 19F MRI nanoprobes for in vivo multicolor imaging to monitor multiple biomolecular or cellular processes.
研究成果
The development of perfluorocarbon-encapsulated silica nanoparticles with distinct 19F NMR peaks enables in vivo multicolor 19F MRI, offering a practical tool for monitoring multiple biomolecular processes and evaluating the effect of surface functional groups on nanoparticle uptake in a single mouse. This technology holds promise for future applications in elucidating biomolecular networks in vivo.
研究不足
The study is limited by the need for further development of nanoprobes with on/off-switching ability in response to various stimuli and the exploration of their application in analyzing the dynamics of multiple enzymes or relationships of enzymes to diverse biological phenomena.
1：Experimental Design and Method Selection:
The study involved the preparation of five types of perfluorocarbon-encapsulated silica nanoparticles (PFC@SiO2) with different chemical shifts for 19F MRI.
2：Sample Selection and Data Sources:
The nanoparticles were characterized using transmission electron microscopy (TEM) and dynamic light scattering (DLS) to confirm their core–shell structures and sizes.
3：List of Experimental Equipment and Materials:
Equipment included an 11 T 19F MRI scanner for imaging and TEM for structural analysis. Materials included various perfluorocarbons and silica nanoparticles.
4：Experimental Procedures and Operational Workflow:
Nanoparticles were prepared and characterized, followed by in vivo multicolor 19F MRI in mice to evaluate their imaging capabilities and the effect of surface functional groups on hepatic uptake.
5：Data Analysis Methods:
The 19F MRI signals were analyzed for sensitivity and specificity, and the hepatic uptake of nanoparticles was quantified based on signal-to-noise ratios.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
