研究目的
Fabrication of a signal-enhanced, label-free, electrochemical immunosensor based on Au nanoparticle/mesoporous silica nanoparticles (Au-MSNs) for NSE determination.
研究成果
The performance of the as-prepared immunosensor was remarkably improved by the modified Au-MSNs, and the sensor could perform sensitive, rapid and selective detection. Specific antigens-antibodies reactions-based immunosensors are potential candidates for ordinary analytical, physiological, biological, clinical and veterinary assays.
研究不足
The poorly conductive silica led to weak electrical signals, which resulted in an undesirable sensitivity and poor limit of detection.
1：Experimental Design and Method Selection:
Synthesis of MSNs and Au-MSN nanocomposites for label-free NSE determination.
2：Sample Selection and Data Sources:
NSE, rabbit anti-NSE polyclonal antibody, and AP-labeled goat anti-rabbit antibody were commercially available.
3：List of Experimental Equipment and Materials:
XRD for structural features, FTIR for verification of Au molecules on silica NPs.
4：Experimental Procedures and Operational Workflow:
Preparation of MSNs, synthesis of Au-MSNs, fabrication of immunosensor, and electrochemical measurements.
5：Data Analysis Methods:
Differential pulse voltammetric (DPV) measurements for NSE detection.
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