研究目的
To separate and identify functional groups of molecules responsible for fluorescence compounds present in biodiesel using column chromatography coupled with infrared spectroscopy and multivariate analysis.
研究成果
The emission spectra of molecular fluorescence allowed identifying molecules such as β-carotene and chlorophyll in soybean oil and biodiesel. Column chromatography followed by infrared spectroscopy and PCA discriminated the fractions by functional groups separation, identifying CH2/CH3 and C=O of carboxylic acid, C=O of ester, and C?O bond as responsible for the separation.
研究不足
The study focused on identifying functional groups responsible for fluorescence in biodiesel but did not quantify the concentration of these fluorophores. The method's effectiveness may vary with different biodiesel sources.
1：Experimental Design and Method Selection:
Column chromatography coupled with infrared spectroscopy and multivariate analysis was performed to separate and identify functional groups of molecules responsible for fluorescence in biodiesel.
2：Sample Selection and Data Sources:
A biodiesel sample (B100) was obtained from the Brazilian Petroleum Corporation (PETROBRAS). Refined soybean oil was purchased from a local supermarket. Patterns used were synthetic β-carotene, α-tocopherol, and linolenic acid.
3：List of Experimental Equipment and Materials:
LED fluorimeter model Q798FIL (Quimis?), FT-IR Spectrometer Frontier (PerkinElmer?), silica gel for column chromatography, and solvents like acetone, hexane, isopropyl alcohol, etc.
4：Experimental Procedures and Operational Workflow:
The biodiesel sample was packed in a chromatographic column, and the fractions obtained were analyzed by FTIR. Before separation, the sample was analyzed by LED-induced fluorescence and compared with β-carotene and soybean oil patterns.
5：Data Analysis Methods:
Principal component analysis (PCA) was used to group the samples and identify the functional groups responsible for the separation.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
