研究目的
Investigating the metabolic and vascular phenotypes of three sibling breast tumor lines with different metastatic potential in vivo with a newly developed quantitative spectroscopy system.
研究成果
The study demonstrates that quantitative optical spectroscopy can capture tumor metabolic phenotypes within a wide dynamic range in murine flank tumor models. This capability will enable studies to understand how the interplay between metabolism and the associated vasculature underlies cancer progression, metastasis, and resistance to therapies.
研究不足
The study is limited to murine breast tumor models and may not fully represent human tumor metabolic and vascular diversity. The optical technique's applicability to other tumor types or in clinical settings requires further validation.
1：Experimental Design and Method Selection:
Utilized a quantitative spectroscopy system to investigate the metabolic and vascular phenotypes of three sibling breast tumor lines with different metastatic potential.
2：Sample Selection and Data Sources:
Female athymic nude mice (nu/nu, NCI, Frederick, Maryland) between 8 to 10 weeks old were used. Mice were assigned to non-tumor, non-metastatic tumor, micro-metastatic tumor, and metastatic tumor groups.
3：List of Experimental Equipment and Materials:
Quantitative optical spectroscopy platform, TMRE (100 μL of 75 μM), 2-NBDG (100 μL of 6 mM 2-NBDG).
4：Experimental Procedures and Operational Workflow:
Mice received optical measurements under isoflurane anesthesia. Tumor length and width were measured using calipers to estimate tumor volume.
5：Data Analysis Methods:
All in vivo optical spectral data were processed using a scalable inverse Monte Carlo (MC) model to extract tissue absorption spectra, and corrected fluorescence of 2-NBDG and TMRE.
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