研究目的
To overcome the limitations of previous frequency-domain USF systems by developing a camera-based USF imaging system with a new scan method, Z-scan, to improve imaging speed and resolution in deep tissue.
研究成果
The camera-based USF imaging system with the Z-scan method significantly improved imaging speed and overcame previous limitations. The USF imaging of contrast agent distribution inside porcine tongue tissue was validated by micro-CT imaging, demonstrating the system's potential for high-resolution deep-tissue imaging.
研究不足
The scan region was only divided into four subregions and was limited by the speed of translation. This limitation can be solved in the future by optimizing the exposure time and improving the speed of scanning the HIFU focus.
1：Experimental Design and Method Selection:
The study proposed a camera-based USF imaging system using an EMCCD camera and a Z-scan method to improve imaging speed and resolution.
2：Sample Selection and Data Sources:
Porcine muscle and tongue tissues were used as samples to mimic human tissue properties.
3：List of Experimental Equipment and Materials:
Included an EMCCD camera, HIFU transducer, function generators, RF power amplifier, matching network, and various filters.
4：Experimental Procedures and Operational Workflow:
The system involved scanning tissue samples with the HIFU transducer, collecting fluorescence images before and after ultrasound heating, and analyzing the increase in fluorescence intensity.
5：Data Analysis Methods:
The increase in fluorescence was extracted by subtracting the background fluorescence image from the post-HIFU exposure image, with further processing to represent the HIFU-induced fluorescence increase.
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Function generator
33500B
Agilent
To drive the excitation laser and synchronize the camera exposure, ultrasound heating and translation stage movement.
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Power and energy meter
PM100D
Thorlabs Inc.
To measure the intensity of the excitation light.
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Micro-CT system
Skyscan 1178
Bruker
To validate the results of the USF imaging.
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EMCCD camera
ProEM-HS:1024BX3
Princeton Instruments
To receive the emitted photons instead of the fiber bundle, improving collection efficiency.
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HIFU transducer
H-108
Sonic Concepts Inc.
To switch on/off the USF contrast agent in its focus.
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RF power amplifier
A075
E&I
To amplify the driving signal generated from the function generator.
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Matching network
To deliver the amplified driving signal to the HIFU transducer.
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Band pass filter
FF01-785/62-25
Semrock Inc.
To filter the excitation light.
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Emission filters
BLP01-830R-50, BLP01-830R-25
Semrock Inc.
To filter the emitted fluorescence signal.
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Camera lens
AF NIKKOR 50mm f/1.8D Lens
Nikon
To focus the emitted fluorescence signal onto the EMCCD camera.
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Data acquisition card
PCIE-6363
National Instruments
To receive triggers from the function generator and send commands to the translation stage.
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Motorized translation stage
Velmex Inc.
To control the movement of the HIFU transducer for scanning the sample.
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