研究目的
To develop a novel nanopillar-assisted SERS chromatography (NPC-SERS) method for simultaneous quantitation of target molecules and analysis of complex, multicomponent fluids, facilitating rapid and quantitative detection of analytes in complex biofluids.
研究成果
The NPC-SERS method successfully segregates and detects PAR and human urine metabolites with a linear dynamic range of 0?500 ppm, offering a novel approach for quantitative detection in complex biofluids.
研究不足
The technique requires optimization of AuNP density for efficient separation and may face challenges in separating components at high analyte concentrations.
1：Experimental Design and Method Selection:
The study combines gold-coated silicon nanopillar (AuNP) SERS substrates with a centrifugal microfluidic platform for automated sample manipulation and spatial separation of molecular species.
2：Sample Selection and Data Sources:
Human urine samples spiked with paracetamol (PAR) were used to demonstrate the method's applicability.
3：List of Experimental Equipment and Materials:
Centrifugal microfluidic disc integrated with AuNP SERS substrates, poly(methyl methacrylate) (PMMA) for disc manufacturing, and ethanol as a surfactant.
4：Experimental Procedures and Operational Workflow:
The process involves sample injection, centrifugation, transfer to a sensing chamber, wicking effect for sample transport on the AuNP surface, and SERS measurements.
5：Data Analysis Methods:
Multivariate curve resolution-alternative least-squares (MCR-ALS) spectral decomposition technique for analyzing SERS maps.
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