研究目的
To develop a novel piezoelectric biosensor for the detection of uric acid (UA) with high sensitivity and selectivity, based on the immobilization of TiO2 nanoparticles and modification with a molecularly imprinted TiO2 layer on a QCM electrode.
研究成果
The fabricated MIT/TiO2 nanoparticle/QCM biosensor demonstrates high sensitivity, selectivity, stability, and reusability for UA detection, with a wide linear range and low detection limit. It is feasible for practical applications in biological samples like urine, showing excellent performance compared to existing methods.
研究不足
The QCM response lacks inherent selectivity, which is addressed by molecular imprinting, but the method may be limited by the need for calcination for reusability and potential interference in complex biological samples. Optimization of pH and template removal could be areas for improvement.
1：Experimental Design and Method Selection:
The study combines surface modification and molecular imprinting techniques to create a piezoelectric sensing platform. TiO2 nanoparticles are immobilized on a QCM electrode, and a molecularly imprinted TiO2 (MIT) layer is formed via sol-gel hydrolysis of Ti(O-nBu)4 in the presence of UA template molecules, followed by calcination to remove templates and crystallize TiO
2：Sample Selection and Data Sources:
Uric acid (UA) and interferents (ascorbic acid, urea, glucose, glutamic acid, purine, cytosine) are used as analytes. Urine samples are diluted and tested without pretreatment.
3：List of Experimental Equipment and Materials:
Equipment includes Q-sense QCM instrument, AT-cut quartz crystals, frequency counter (Iwatsu SC-7201), XRD (Bruker-AXS D8), FTIR spectrometer (Nicolet 200SXV), SEM (Hitachi S-520). Materials include Ti(O-nBu)4, TiO2 nanoparticles (~100 nm), UA, and other chemicals from specified suppliers.
4：0). Materials include Ti(O-nBu)4, TiO2 nanoparticles (~100 nm), UA, and other chemicals from specified suppliers. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: TiO2 nanoparticles are attached to QCM electrode via spin coating and heat treatment. MIT layer is formed by dipping in sol-gel solution, rinsing, hydrolyzing, drying, and calcining. QCM measurements are performed at 25°C in PBS buffer, with UA solutions injected, and frequency changes recorded.
5：Data Analysis Methods:
Frequency responses are monitored and analyzed for sensitivity, linear range, detection limit, selectivity against interferents, reusability, and stability. Statistical analysis includes standard deviation for recovery tests.
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Frequency counter
SC-7201
Iwatsu
Monitoring frequency changes
-
X-ray diffractometer
D8
Bruker-AXS
Crystal structure analysis
-
Scanning electron microscope
S-520
Hitachi
Surface morphology imaging
-
Q-sense instrument
Not specified
Q-sense
Piezoelectric measurements for mass sensing
-
Quartz crystals
AT-cut, 9-MHz, diameter 12 mm
Beijing Chenjing Co
QCM resonator for sensing
-
FTIR spectrometer
200SXV
Nicolet
Infrared spectrum recording
-
TiO2 nanoparticles
ca 100 nm mean particle diameter
Aladdin Reagent Co
Carrier material for sensing layer
-
Ti(O-nBu)4
Not specified
Sigma Chem Co
Precursor for sol-gel hydrolysis
-
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