研究目的
To synthesize highly luminescent silicon nanoparticles using a green reducing agent (Citrus limon) and apply them in luminescent cell imaging and antimicrobial efficacy studies.
研究成果
The study successfully synthesized water-dispersible silicon nanoparticles using a green reducing agent, demonstrating strong luminescence suitable for cell imaging and antimicrobial properties at concentrations above 1.5 μg/ml, with potential applications in biomedical fields.
研究不足
The synthesis may lead to agglomeration if purified by centrifugation; cytotoxicity and antimicrobial effects are concentration-dependent, requiring careful control; quantum yield is lower compared to some doped nanoparticles.
1：Experimental Design and Method Selection:
One-pot synthesis method using silane precursors (VTMS and APTES) with reducing agents (sodium ascorbate and Citrus limon extract) at ambient conditions with stirring.
2：Sample Selection and Data Sources:
Silane precursors purchased from Sigma Aldrich and Loba Chemie; Citrus limon obtained locally; microbial strains from MTCC-IMTECH; human white blood cells and mice epithelial cells used for imaging.
3：List of Experimental Equipment and Materials:
Transmission electron microscope (Technai), UV-Vis spectrophotometer (Perkin Elmer Lambda 750), PL spectrometer (Perkin Elmer LS55), FTIR spectrometer (Bruker), inverted microscope (Leica DM IL LED), and various chemicals.
4：Experimental Procedures and Operational Workflow:
Synthesis involved adding silane precursors to deionized water with reducing agents, stirring for 25 minutes; antimicrobial tests using disc diffusion method; cell imaging by incubating cells with Si NP and observing under microscope.
5：Data Analysis Methods:
TEM for size and composition, UV-Vis and PL for optical properties, FTIR for functional groups, and statistical analysis for antimicrobial efficacy.
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UV-Vis Spectrophotometer
Lambda 750
Perkin Elmer
Recording UV-Vis absorption spectra of silicon nanoparticles.
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PL Spectrometer
LS55
Perkin Elmer
Recording photoluminescence spectra of silicon nanoparticles.
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Inverted Microscope
DM IL LED
Leica
Acquiring luminescence images of cells after silicon nanoparticle internalization.
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Transmission Electron Microscope
Technai
Obtaining TEM micrographs and EDX spectra for size and composition analysis of silicon nanoparticles.
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FTIR Spectrometer
Bruker
Recording FTIR spectra to identify functional groups in silicon nanoparticles.
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APTES
>97%
Sigma Aldrich
Used as a silicon precursor in the synthesis of silicon nanoparticles.
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VTMS
>98%
Sigma Aldrich
Used as a silicon precursor in the synthesis of silicon nanoparticles.
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Sodium Ascorbate
>99.9%
Loba Chemie
Used as a chemical reducing agent in the synthesis of silicon nanoparticles.
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