研究目的
To develop a thermodynamic cancer-therapeutic modality that uses oxygen-irrelevant free radicals generated from thermo-labile initiators triggered by photonic NIR-II laser irradiation for effective cancer treatment in both normoxic and hypoxic conditions.
研究成果
The research successfully established a photonic thermodynamic therapy strategy using AIPH@Nb2C@mSiO2 NPs, which generated oxygen-independent free radicals upon NIR-II laser irradiation, leading to effective cancer cell death in vitro and complete tumor eradication in vivo without recurrence, demonstrating high biosafety and potential for multimodal imaging-guided therapy.
研究不足
The study may have limitations in scalability for clinical translation, potential long-term toxicity not fully assessed, and the need for further optimization of the nanoplatform for human applications.
1：Experimental Design and Method Selection:
The study designed a core/shell nanostructure (Nb2C@mSiO2) to encapsulate AIPH initiators, utilizing the photothermal properties of Nb2C MXene for thermal-triggered free radical generation. Methods included synthesis, characterization, in vitro and in vivo assays.
2：Sample Selection and Data Sources:
Used 4T1 breast cancer cells and tumor-bearing mice models. Data were obtained from laboratory experiments including cell culture, animal studies, and various imaging techniques.
3：List of Experimental Equipment and Materials:
Included transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), UV-vis spectroscopy, electron spin resonance (ESR) spectrometer, flow cytometry, confocal laser scanning microscope (CLSM), infrared thermal camera, photoacoustic imaging system, and inductively coupled plasma optical emission spectrometer (ICP-OES). Materials involved Nb2AlC MAX-phase ceramic, HF, TPAOH, CTAC, TEOS, AIPH, POBN, ABTS, DCFH-DA, Calcein-AM, PI, Annexin V-FITC, and various chemicals for assays.
4：Experimental Procedures and Operational Workflow:
Synthesized Nb2C nanosheets via HF etching and TPAOH exfoliation, coated with mesoporous silica, loaded with AIPH. Conducted photothermal measurements, free radical generation tests, cellular uptake studies, cytotoxicity assays, apoptosis detection, in vivo imaging, and therapeutic efficacy evaluations in mice.
5：Data Analysis Methods:
Employed statistical analysis for cell viability, flow cytometry for apoptosis, ESR for radical detection, UV-vis for absorbance, and imaging software for quantitative analysis of PA and fluorescence signals.
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Electron Spin Resonance Spectrometer
Bruker X-band A200
Bruker
Used to detect free radicals generated from the nanoparticles.
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Transmission Electron Microscope
Used for imaging and characterizing the nanostructures, such as Nb2C nanosheets and Nb2C@mSiO2 NPs.
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X-ray Photoelectron Spectrometer
Used to determine the chemical composition of the nanoparticles.
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UV-Vis Spectrophotometer
Used for absorbance measurements and quantification of AIPH loading.
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Flow Cytometer
Used for analyzing cell apoptosis and cellular uptake.
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Confocal Laser Scanning Microscope
Used for intracellular imaging and free radical detection.
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Infrared Thermal Camera
Used to monitor temperature changes during photothermal experiments.
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Photoacoustic Imaging System
Used for in vitro and in vivo PA imaging.
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Inductively Coupled Plasma Optical Emission Spectrometer
ICP-OES
Used for pharmacokinetic and metabolism studies by detecting Nb and Si concentrations.
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1064 nm NIR-II Laser
Used for photothermal triggering and free radical generation.
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