研究目的
To construct an electrochemiluminescent (ECL) sensor with improved accuracy for the detection of Cu2+ by utilizing a reaction system for dual suppression of ECL recovery.
研究成果
The ECL sensor with dual suppression mechanism shows improved accuracy, high sensitivity (detection limit of 5 nM), and specificity for Cu2+ detection, making it promising for biomedical applications such as serum analysis.
研究不足
The sensor is specific to Cu2+ and may have limitations in complex matrices; optimization of reaction conditions is required for different applications.
1：Experimental Design and Method Selection:
The methodology involves synthesizing Au-CNNS nanohybrids, modifying glassy carbon electrodes, and using a reaction system with NH3/NH4Cl buffer, KSCN, and H2O2 to achieve Cu2+ dose-dependent dual suppression of ECL recovery. ECL responses are recorded using a three-electrode system.
2：Sample Selection and Data Sources:
Au-CNNS nanohybrids are synthesized with varying Au content. Human serum samples from healthy people are used for applicability testing after pretreatment with HNO3 and dilution.
3：List of Experimental Equipment and Materials:
Equipment includes a tube furnace (GSL 1400X, Kejing Materials Technology Lt. Co.), electronic microscope (TecnaiG2 F20S-TWIN 200 kV), ECL detection system (MPI-E, Remex Electronic Instrument Lt. Co.), and ICPMS system (Agilent 7500, Agilent Technologies). Materials include dicyanamide, HAuCl4·3H2O, metal salts, K2S2O8, NaBH4, H2O2, ammonium hydroxide, KSCN, ammonium chloride, and doubly distilled water.
4：Experimental Procedures and Operational Workflow:
Au-CNNS nanohybrids are synthesized and used to modify GC electrodes. The reaction system is prepared and optimized. Electrodes are immersed in the reaction system with varying Cu2+ concentrations, rinsed, and ECL responses are recorded in PBS with K2S2O8 by scanning potential from 0 to -1.1 V.
5：1 V. Data Analysis Methods:
5. Data Analysis Methods: ECL intensity is measured and compared. Calibration curves are plotted, and selectivity is tested against other metal ions. Data from serum samples are compared with ICP-MS results.
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ICPMS system
7500
Agilent Technologies
Used to detect Cu2+ concentration in serum samples.
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tube furnace
GSL 1400X
Kejing Materials Technology Lt. Co.
Used for synthesizing bulk g-CN by heating.
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electronic microscope
TecnaiG2 F20S-TWIN
Recorded TEM images of Au-CNNS nanohybrids.
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ECL detection system
MPI-E
Remex Electronic Instrument Lt. Co.
Employed for recording electrochemical and ECL responses.
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glassy carbon electrode
Used as the working electrode in the three-electrode system.
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Pt wire
Used as the counter electrode.
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Ag/AgCl electrode
Used as the reference electrode.
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photomultiplier tube
PMT
Used in ECL detection, set at 800 V potential.
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