研究目的
Developing a highly selective fluorescent probe for sequential sensing of copper(II) and iodide ions in acetonitrile.
研究成果
The dansylamido-thiazole fluorescent probe (1) selectively recognizes Cu2+ and iodide ions sequentially in acetonitrile through chelation-induced inhibition of ESIPT and ICT processes, followed by reduction and partial fluorescence restoration. It demonstrates high sensitivity and selectivity, with potential applications in optical sensing, though further optimization for complete signal recovery and broader solvent compatibility is needed.
研究不足
The experiments were conducted only in acetonitrile solution, which may limit applicability to other solvents or real-world samples. The fluorescence restoration with iodide is partial due to factors like resonance energy transfer and stabilization of Cu+ ions, indicating potential interference or incomplete sensing. Selectivity was tested against a set of ions but not exhaustively in complex matrices.
1：Experimental Design and Method Selection:
The study involved designing a fluorescent probe (probe 1) based on a dansyl-thiazole conjugate for ion sensing. UV-Vis and fluorescence spectroscopy were used to study binding and recognition abilities. Theoretical models include ESIPT, ICT, and metal-ligand electron transfer mechanisms.
2：Sample Selection and Data Sources:
Probe 1 and model compound 2 were synthesized and characterized. Solutions were prepared in acetonitrile (CH3CN) with concentrations in the μM range. Metal cations (e.g., Cu2+, Na+, K+) and anions (e.g., I?, F?, Cl?) were added as perchlorate or tetrabutylammonium salts.
3：List of Experimental Equipment and Materials:
Spectrophotometers for UV-Vis and fluorescence measurements, NMR spectrometer for structural characterization, hydrogenation apparatus with Pd/C catalyst, column chromatography for purification. Materials include acetonitrile, dansyl chloride, triethylamine, dichloromethane, ethanol, various metal perchlorates, and tetrabutylammonium iodide.
4：Experimental Procedures and Operational Workflow:
Synthesis of probes via hydrogenation and reaction with sulfonyl chlorides. Titration experiments: adding aliquots of metal ions or anions to probe solutions, measuring spectral changes. Job's plot and binding constant calculations were performed. 1H NMR studies to investigate sensing mechanisms.
5：Data Analysis Methods:
Non-linear regression analysis for binding constants, Job's plot for stoichiometry determination, spectral analysis for shifts and quenching/enhancement.
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