研究目的
To propose and demonstrate a temporally low-coherent optical diffraction tomography technique using angle-scanning Mach-Zehnder interferometry to address coherent noise issues in quantitative phase imaging.
研究成果
The study successfully demonstrates a low-coherent ODT technique that mitigates coherent noise through diffractive modulation and careful bandwidth selection. It provides 3D refractive index imaging of biological samples but highlights fundamental limitations in axial resolution and practical challenges, suggesting optimal use with low-coherent light for maximum quality.
研究不足
Limitations include the nondispersive assumption for samples, poor axial resolution in transmission geometry due to thin axial thickness in k-space, illumination angle limitations with broadband light, and reduced signal-to-noise ratio at higher lateral frequencies. The method is less effective for highly absorptive samples and may have artifacts from sample debris or coverslip stains.
1：Experimental Design and Method Selection:
The study uses a Mach-Zehnder interferometer setup with diffractive modulation via a digital micromirror device (DMD) to maintain interference during angle scanning. The reconstruction principles for incoherent ODT are reviewed and applied, incorporating assumptions like nondispersive samples.
2：Sample Selection and Data Sources:
Samples include polystyrene microspheres (10 μm diameter), human red blood cells from a healthy donor, and rat pheochromocytoma (PC-12) cells cultured for 24 hours. Data is acquired through interferometric measurements.
3：List of Experimental Equipment and Materials:
Equipment includes a supercontinuum light source (EXR-4, NKT Photonics Inc.), bandpass filter, digital micromirror devices (DLP LightCrafter 6500, Texas Instruments Inc.), diffraction grating (GT13-03, Thorlabs Inc.), microscope body (IX71, Olympus Inc.), objective lens (UPLSAPO 60XW, Olympus Inc.), condenser lens (UPLSAPO 60XW, Olympus Inc.), monochromatic camera (MD120MU-SY, XIMEA GmbH), and translation stage. Materials include immersion oil (RI=1.561), Alservier's solution (A3551, Sigma-Aldrich Inc.), and cell culture reagents.
4：561), Alservier's solution (A3551, Sigma-Aldrich Inc.), and cell culture reagents. Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The setup involves matching optical path lengths, using DMDs for diffractive modulation to compensate for chromatic dispersion, acquiring interferograms with the camera synchronized to the illumination, and performing angle scanning with structured illumination patterns. Interferograms are processed to retrieve optical fields, and ODT reconstruction is applied using Rytov approximation and regularization techniques.
5：Data Analysis Methods:
Data analysis includes retrieving scattered fields from interferograms, applying weight functions based on spectral density, and reconstructing 3D refractive index distributions using edge-preserving regularization to handle missing cone issues.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
supercontinuum source
EXR-4
NKT Photonics Inc.
Provides spatially coherent broadband light for illumination in the interferometric setup.
暂无现货
预约到货通知
-
diffraction grating
GT13-03
Thorlabs Inc.
Introduced in the reference arm for diffractive modulation to prevent decoherence.
暂无现货
预约到货通知
-
microscope body
IX71
Olympus Inc.
Commercial microscope used as the base for the optical setup.
暂无现货
预约到货通知
-
objective lens
UPLSAPO 60XW
Olympus Inc.
Used for imaging with high numerical aperture.
暂无现货
预约到货通知
-
condenser lens
UPLSAPO 60XW
Olympus Inc.
Used for illumination with high numerical aperture.
暂无现货
预约到货通知
-
monochromatic camera
MD120MU-SY
XIMEA GmbH
Used for acquiring interferograms synchronized with the illumination.
立即获取同款设备 -
digital micromirror device
DLP LightCrafter 6500
Texas Instruments Inc.
Used for diffractive modulation in the illumination unit to maintain interference during angle scanning.
暂无现货
预约到货通知
-
bandpass filter
Used to minimize issues from broad source bandwidth by filtering the light.
暂无现货
预约到货通知
-
translation stage
Used to match optical path lengths in the reference arm.
暂无现货
预约到货通知
-
登录查看剩余7件设备及参数对照表
查看全部
